Restriction Enzyme Worksheet 1 Answer Key Assessment Questions Answer Key 1 Describe the role of restriction enzymes in the process of transformation Restriction enzymes are used to cut the DNA of both the organism with the desired gene and the plasmid This allows the fusion of the nitrogen base pairs of the two DNA segments 2 The restriction enzyme BamH1 cuts DNA between the two
Restriction enzymes which are produced naturally by bacteria cut DNA molecules at specific sites denoted by specific base sequences Approximately 3000 different restriction enzymes have been identified and each cuts at different base sequences A restriction digestion occurs when a restriction enzyme cuts DNA molecules The size of the fragments Created Date 20160316070915Z
Restriction Enzyme Worksheet 1 Answer Key
Restriction Enzyme Worksheet 1 Answer Key
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1 Below is a restriction map for the plasmid pGEN101 total length 20 kb Using this map as a guide give the number of restriction fragments along with their associated lengths that would result from digesting pGEN101 with the restriction enzymes EcoRI BamHI and a combination of EcoRI BamHI 6kb BamHI After this lesson students should be able to Describe the basic steps involved in DNA fingerprinting Explain the function of restriction enzymes and the role of restriction sites along the DNA Explain how DNA fragments are charged molecules Explain that the distance traveled by DNA fragments inside a gel tray depends on fragment length
Teacher s Guide Key This assignment is usually given with the unit on biotechnology Biotechnology Notes and Powerpoint Presentation Who Ate the Cheese use restriction enzymes to cut DNA and place on a chart to simulate movement of fragments during eletrophoresis Recombinant DNA simulation cut human and bacteria DNA at specific points Created Date 3 2 2016 8 39 37 AM
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Overview Students learn about restriction enzymes and how they work through this lesson First they will use an interactive website to see how the restriction enzymes locate their cut sites on the DNA and then actually cut the DNA so it can be combined with other DNA to make a recombinant organism Then they use two additional worksheets to Mini Lesson 2 Gel Electrophoresis Answer the following questions based upon the diagram of the gel seen below 1 Label the positive and negative electrode side of the gel 2 Draw an arrow on the gel in the direction the DNA will move and explain why it will move in that direction 3 Label the wells from left to right with the following symbols
DNA Technology Worksheet MCQ Carbohydrates questions and answers Gabriela Leon TCD Biochem Exam 2 Ruiwen Lin1 High concentraion of enzyme reason for partial cleavage Study with Quizlet and memorize flashcards containing terms like Isoschizomers Neoschizomers Star activity and more Compare restriction enzyme differences on identical DNA Directions Identify the restriction sites for each of the examples given Show the cuts sticky Microsoft Word Restriction Enzymes Worksheet doc Author user Created Date 6 26 2008 1 23 55 PM
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Restriction Enzyme Worksheet 1 Answer Key - 1 Below is a restriction map for the plasmid pGEN101 total length 20 kb Using this map as a guide give the number of restriction fragments along with their associated lengths that would result from digesting pGEN101 with the restriction enzymes EcoRI BamHI and a combination of EcoRI BamHI 6kb BamHI